Lactating mice pass along common antimicrobial to pups, initiating liver damage ucsandiego NatureComms
. Representative images were captured on an upright light/fluorescent microscope equipped with AxioCam camera.
For the staining of F4/80 and Ki-67 , paraffin liver sections were prepared in the Histology Core . Formalin-fixed, paraffin-embedded liver slides were deparaffinized and rehydrated, using xylene followed by alcohol and PBS washings. Antigen retrieval of tissue slides and the immunohistochemical staining with a primary antibody, secondary biotinylated antibody, and streptavidin-HRP were achieved as described previously.
Detection of apoptotic cells in tissue sections was performed by the TUNEL assay with the In-Situ Cell Death Detection Kit , according to the protocol described previously. TUNEL-positive cells were counted on five fields of four different mice of each group of ×200 magnification per slide. For Sirius Red and F4/80 quantification, images were converted to RGB stack and middle section was used.
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