A Nature research paper reports that a microRNA encoded by a human herpesvirus inhibits a host microRNA and leads to mitochondrial fragmentation, reduced interferon response and virus reactivation from latency
mRNA levels were studied in the same samples as in Fig. a and b respectively. Cq values for mature miRNA amplification were normalized with U6 snRNA. Cq values for pri-/pre-miRNAs were normalized with beta actin. Furthermore, fold change values of virus infected samples were normalized against the same from mock infected samples and TSA treated samples were normalized against mock treated samples.Cq values were normalized aginst beta actin. Data are mean ± SD.
Doxycycline--inducible wild-type and mutant miR-aU14 shRNAs were expressed in HeLa cells under control of a dox-inducible Pol III promoter. Mature miR-aU14 sequence are indicated in red. Point mutations are highlighted in blue within a black rectangle.. HeLa cells were stably transduced to express dox-inducible miR-aU14. miR-30c and miR-aU14 expression was probed by Northern blotting. Probe against human U6 served as loading control.
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