This article discusses techniques for improving the signal-to-noise ratio in flow cytometer optics, emphasizing the importance of optimizing optical components and configurations to enhance the accuracy of flow cytometry measurements.
Sponsored Content by Avantier Inc.Reviewed by Andrea SalazarJun 21 2024 Flow cytometer SNR This article will review flow cytometry to help readers understand the challenges faced when increasing flow cytometer SNR .
A detector then collects the emission from the excited fluorophores in addition to the side and forward scatter, which is then converted to a photocurrent and recorded by the system.Forward scatter detection occurs when the lasers are directly pointed at a detector. In contrast, side scatter detection involves using a detector configured in a side placement, and the lenses are positioned to route the side-scattered light to the detector.
Output power stability: How high are the time-dependent variations in the laser output power? Two values determine the strength of the laser power or potential lack thereof: peak-to-peak and root mean square, or rms . Filters, mirrors, and lenses A chain is only as strong as its weakest link, and even the most superior of lasers with optimal output power stability and laser beam quality will not come close to producing high SNR if subpar optics are being used.
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