Nature research paper: Columnar structure of human telomeric chromatin
Extended Data Fig. 8 Contribution of histone tails to stabilisation of the columnar structure of the telomeric chromatin fibre., AUC-SV data for the Telo-tetra arrays with intact histone tails and with the C-terminal truncated H2A ) in the unfolded or compacted forms. In the absence of Mg, the s-value distribution of both Telo-tetra fibres shows two peaks. We identified the peak at the larger and lower s-values as tetramers and under-saturated fibres .
Extended Data Fig. 9 Digestions of Telo-tetra and structural analysis of the NRL in telomeric tetranucleosome., AvaI, MNase and DNase digestion of the Telo-tetra. Lane M is a DNA marker; two lanes marked “Ar” show Telo-tetra. The left “Ar” shows Telo-tetra in low salt buffer, and the right lane shows Telo-tetra in DNase buffer. Lanes “AvaI”, “MN”, and “DN” are respectively digestion of the Telo-tetra by AvaI endonuclease, Micrococcal nuclease , and DNase I nuclease.
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