Chinese researchers claim to have created a new gene-editing technique called CyDENT that is more effective than Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology.
In some cases, not all cells within a treated organism or tissue may undergo the desired gene editing, leading to mosaicism, a situation where a person has two or more genetically different sets of cells in his or her body. Because some cells contain the desired genetic changes while others do not, this can make it more difficult to use certain therapeutic procedures.
Finally, there is a chance that when CRISPR is used for gene therapy in people, the immune system would mistake the Cas9 protein for something foreign and launch an immunological reaction, which could limit how effective the treatment is. Zhao further explained that CRISPR-based systems depend on a guide RNA to function, which may not be a problem when editing the DNA in a cell's nucleus but becomes one when modifying the DNA in a plant's chloroplast or a human mitochondria. Guide RNA is a piece of RNA designed by researchers to use Cas9 for gene editing.
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