Watch a virus in the moments right before it attacks naturemethods
A key factor to integrating the tracking and imaging modules in 3D-TrIm is minimization of crosstalk between the channels. In this implementation, tracking fluorescence collection occurs in the 500–600-nm window and imaging fluorescence is collected in the 600–700-nm window.
After DCM3, the tracking emission is relayed by a 300-mm lens , placed 625 mm beyond the previous 85-mm lens, and focused onto an APD by an 80-mm lens . This detection setup results in a beam magnification that nearly fills the APD sensor area and prevents interference from the scanned imaging laser.
The autopilot algorithm searches for particles and considers any particle-detection event as a trajectory regardless of duration. After a set duration, either time- or quantity-based, an area-migration event is triggered, which moves the motorized stage to a new location in an S pattern to minimize overall distance traveled. The algorithm then continues to search for particles in the new area and repeats this process until it is manually terminated, or an imposed time limit has been reached.
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