Nature research paper: Brown-fat-mediated tumour suppression by cold-altered global metabolism
values and migration times of corresponding authentic standard compounds. Data were quantified by comparing the ratio of analyte peak area/internal standard peak area of sample solution to that of the reference standard mixture. The quantitative formula was as follows:= / × × ) × 1/ × )/). The sample relative area = /. The standard relative area = /. Chromatograms of each metabolite were extracted and quantified using the Agilent MassHunter Qualitative Analysis software .
Mice in thermoneutral and cold environments were anaesthetized and kept at room temperature for 5 min for imaging. Thermal images on the back of mice were captured using an infrared thermal imager . Setting environmental temperature as the baseline, thermal images of mice were acquired and were further analysed using Fotric AnalyzIR.The paraffin-embedded tissue sections of 5 μm thickness were incubated at 60 °C for approximately 2 h to melt the covered paraffin.
Total RNA was extracted from cells and tissues using the TRIzol and GeneJET RNA Purification Kits according to the manufacturer’s instructions. The total RNA was reverse-transcribed and cDNAs were used for PCR and qPCR analyses using the primers listed in Supplementary Table. Samples were stored at −20 °C and processed for qPCR using the ABI Prism 7500 System .
For patients with cancer, an 18-year-old female patient with Hodgkin’s lymphoma participated in a pilot study during the intervals of the fifth cycle of combination chemotherapy of adriamycin, bleomycin, vinblastine and dacarbazine for 5 cycles. The cold ambient temperature was approximately 22 °C regulated by air-conditioning for 1 week and the patient wore light clothing. The patient fasted overnight before PET–CT examination.
Canada Latest News, Canada Headlines
Similar News:You can also read news stories similar to this one that we have collected from other news sources.
Human Activities Affect Elephants’ Use of Nature PreservesA study shows that elephants’ activity in these areas varies, likely according to their perceptions of the threat of human activities.
Read more »
Nature’s Flower Show: Check Out the Swamp Rose Mallow at Sand Ridge Nature CenterThe native swamp rose mallow has hit full bloom, its super-sized six-inch blossoms bursting forth in seemingly every shade of pink from the palest blush to saturated fuschia
Read more »
The BioInnovation Institute as a catalyst for European life science startup creation - Nature BiotechnologyNature Biotechnology - The BioInnovation Institute as a catalyst for European life science startup creation
Read more »
Multiple exciton generation in tin–lead halide perovskite nanocrystals for photocurrent quantum efficiency enhancement - Nature PhotonicsResearchers demonstrate low-threshold multiple exciton generation and photocurrent quantum efficiency exceeding 100% from high-energy photons in perovskite nanocrystals.
Read more »
Anti-inflammatory clearance of amyloid-β by a chimeric Gas6 fusion protein - Nature MedicineAn engineered protein engages the efferocytosis pathway to induce amyloid-β engulfment, resulting in behavioral rescue in Alzheimer’s disease mouse models without the increased inflammation or vascular pathology associated with conventional antibody therapy
Read more »
The digital phenotype of vaccination - Nature BiotechnologyNature Biotechnology - The digital phenotype of vaccination
Read more »