Researchers discover that various species share a similar mechanism of molecularresponse to nanoparticles NatureNano
BEAS-2B cells were grown in BEGM . Cells were cultivated in 75 cm. For all experiments, 500 µl of cells was seeded at a density of 3.75 × 10cells per ml in 48-well plates. Cells were then left to rest overnight before transfections. One hour before transfection, the media was replaced with 225 µl of fresh BEGM per well. Two vectors were used for transfection: human cytomegalovirus and the ZNF362, created with VectorBuilder . Per well, 0.
The Dual-Glo luciferase Assay System was used as per the manufacturer’s guidelines to measure firefly and renilla luciferase activity, on a Spark multiplate reader . There were three samples measured for each vector and vehicle control, for each exposure. The mean signal of three background wells was used to subtract background from luciferase measurements. The firefly luciferase activity was normalized to renilla luciferase and power transformed. When present, outliers were removed with the boxplot function in R.
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